M-PER™ Mammalian Protein Extraction Reagent

Protein isolation Tissue - Human aortic endothelial cells

Experiment
Protein isolation Tissue - Human aortic endothelial cells
Product
M-PER™ Mammalian Protein Extraction Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Brain tissue was lysed in Mammalian Protein Extraction Reagent (M-PER) with phosphatase and protease inhibitors (10 μl/ml, Halt™ Protease and Phosphatase Inhibitor Cocktail, Thermo Scientific Inc., Rockford, IL, USA) using sonication, left to incubate on ice for 10 min and centrifuged at 20,000g at 4 °C for 20 min. The supernatant was taken, and centrifuged at 9000g at 4 °C for 5 min. Supernatant was taken again and used for further analysis.

Publication protocol

Brain tissue was lysed in Mammalian Protein Extraction Reagent (M-PER) with phosphatase and protease inhibitors (10 μl/ml, Halt™ Protease and Phosphatase Inhibitor Cocktail, Thermo Scientific Inc., Rockford, IL, USA) using sonication, left to incubate on ice for 10 min and centrifuged at 20,000g at 4 °C for 20 min. The supernatant was taken, and centrifuged at 9000g at 4 °C for 5 min. Supernatant was taken again and used for further analysis.

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Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Human aortic endothelial cells using M-PER™ Mammalian Protein Extraction Reagent from Thermo Fisher Scientific.

Paper title
Disruption of normal circadian clock function in a mouse model of tauopathy.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for M-PER™ Mammalian Protein Extraction Reagent below.

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Videos

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