M-PER™ Mammalian Protein Extraction Reagent

Protocol tips

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	Brain tissue was lysed in Mammalian Protein Extraction Reagent (M-PER) with phosphatase and protease inhibitors (10 μl/ml, Halt™ Protease and Phosphatase Inhibitor Cocktail, Thermo Scientific Inc., Rockford, IL, USA) using sonication, left to incubate on ice for 10 min and centrifuged at 20,000g at 4 °C for 20 min. The supernatant was taken, and centrifuged at 9000g at 4 °C for 5 min. Supernatant was taken again and used for further analysis.

Protocol tips

Brain tissue was lysed in Mammalian Protein Extraction Reagent (M-PER) with phosphatase and protease inhibitors (10 μl/ml, Halt™ Protease and Phosphatase Inhibitor Cocktail, Thermo Scientific Inc., Rockford, IL, USA) using sonication, left to incubate on ice for 10 min and centrifuged at 20,000g at 4 °C for 20 min. The supernatant was taken, and centrifuged at 9000g at 4 °C for 5 min. Supernatant was taken again and used for further analysis.

Publication protocol

Brain tissue was lysed in Mammalian Protein Extraction Reagent (M-PER) with phosphatase and protease inhibitors (10 μl/ml, Halt™ Protease and Phosphatase Inhibitor Cocktail, Thermo Scientific Inc., Rockford, IL, USA) using sonication, left to incubate on ice for 10 min and centrifuged at 20,000g at 4 °C for 20 min. The supernatant was taken, and centrifuged at 9000g at 4 °C for 5 min. Supernatant was taken again and used for further analysis.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for M-PER™ Mammalian Protein Extraction Reagent below.

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