RIPA Lysis Buffer (ProteinSimple)
Protein isolation Tissue - Mouse aorta
Publication protocol
The aortas obtained from mice in each groups were homogenized with 100 μL RIPA Lysis Buffer. The homogenate was placed on ice for 1 h then centrifuged at 15000 for 15 minutes at 4°C. The supernatant was collected and denatured in boiling water for 8 min. The protein samples were stored at −20°C until further analysis.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
When should I be harvesting the tissue before using it for the most optimal results?
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
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