RIPA Lysis Buffer (ProteinSimple)

Protein isolation Tissue - Mouse aorta

Experiment
Protein isolation Tissue - Mouse aorta
Product
RIPA Lysis Buffer (ProteinSimple) from ProteinSimple
Manufacturer
ProteinSimple

Protocol tips

Protocol tips
- Instructions are followed regarding the manufacturer's protocol.

Publication protocol

The aortas obtained from mice in each groups were homogenized with 100 μL RIPA Lysis Buffer. The homogenate was placed on ice for 1 h then centrifuged at 15000 for 15 minutes at 4°C. The supernatant was collected and denatured in boiling water for 8 min. The protein samples were stored at −20°C until further analysis.

Full paper   Login or join for free to view the full paper.

Reviews

RIPA Lysis Buffer (ProteinSimple) from ProteinSimple has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse aorta using RIPA Lysis Buffer (ProteinSimple) from ProteinSimple.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from ProteinSimple for RIPA Lysis Buffer (ProteinSimple) below.

We haven't found the manufacturer protocol for this product yet.

Videos

Check out videos that might be relevant for performing Protein isolation Tissue - Mouse aorta using RIPA Lysis Buffer (ProteinSimple) from ProteinSimple. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms