NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Protocol tips

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	- Isolation was performed according the manufacturer's protocol.

Protocol tips
- Isolation was performed according the manufacturer's protocol.

Publication protocol

Additionally, a commercial cell fractionation kit designed to yield near pure nuclei and cytoplasmic fractions from the same starting sample of cells and soft tissues was used (Thermo NE-PER nuclear and cytoplasmic extraction kit, Pierce-Thermo, Northumberland, UK, to obtain a mitochondrial fraction required the use of an additional kit) as directed by the manufacturer. This enabled a comparison of the efficacy of the commercial kit with the method described here when both methods were used to fractionate an identical starting sample (myoblast cells from a T75 cm2 flask, approximately 2×106 cells).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for NE-PER™ Nuclear and Cytoplasmic Extraction Reagents below.

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