T-PER™ Tissue Protein Extraction Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The other part of the cornea was used for protein extraction. The corneas were weighed and separately chopped into small pieces. The tissue was solidified by immersion in liquid nitrogen and then ground into a powder. This step was repeated three times. Tissue extraction was performed according to the reagent instructions (Tissue Protein Extraction Cocktail, ThermoFisher Scientific, Inc., Waltham, MA, USA).

Protocol tips

The other part of the cornea was used for protein extraction. The corneas were weighed and separately chopped into small pieces. The tissue was solidified by immersion in liquid nitrogen and then ground into a powder. This step was repeated three times. Tissue extraction was performed according to the reagent instructions (Tissue Protein Extraction Cocktail, ThermoFisher Scientific, Inc., Waltham, MA, USA).

Publication protocol

The other part of the cornea was used for protein extraction. The corneas were weighed and separately chopped into small pieces. The tissue was solidified by immersion in liquid nitrogen and then ground into a powder. This step was repeated three times. Tissue extraction was performed according to the reagent instructions (Tissue Protein Extraction Cocktail, ThermoFisher Scientific, Inc., Waltham, MA, USA). The tissue extract was collected and the protein content was analyzed by a Coomassie protein assay (ThermoFisher Scientific) to normalize the protein content of the samples for subsequent enzyme linked immunosorbent assay (ELISA). Total protein (15 μg in 100 μL) was loaded into 96-well plates for ELISA. Rabbit TNF-α level was determined using the DY5670 kit from R&D systems (Minneapolis, MN, USA), whereas rabbit IL-8 and IL-6 levels were determined by using kits MBS2507990 and MBS812867 (MyBioSource, San Diego, CA, USA), respectively, according to the supplier’s instructions.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Rabbit eye retina/choroids using T-PER™ Tissue Protein Extraction Reagent from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for T-PER™ Tissue Protein Extraction Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Tissue - Rabbit eye retina/choroids using T-PER™ Tissue Protein Extraction Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.