AllPrep DNA/RNA/Protein Mini Kit

Protein isolation Tissue - Human placental tissue

Experiment
Protein isolation Tissue - Human placental tissue
Product
AllPrep DNA/RNA/Protein Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- Proteins are extracted with a Qiagen AllPrep DNA/RNA/Protein mini kit. Tissues are homogenized following the manufacturer’s instructions with two modifications: (i) addition of protease inhibitors to the lysis buffer (RLT) and (ii) the re-suspension of protein pellets in Modified Reswell Solution.

Publication protocol

Proteins were extracted from placentae with a Qiagen AllPrep DNA/RNA/Protein mini kit (Qiagen Ltd., Crawley, UK), see Ref. [20]. Tissues were homogenized following the manufacturer’s instructions with two main modifications: (i) the addition of protease inhibitors (Protease Inhibitor Cocktail, Sigma-Aldrich Company Ltd., Gillingham, UK) to the lysis buffer (RLT) to maximize protein integrity and yield and (ii) the re-suspension of protein pellets in Modified Reswell Solution [21].

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Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Human placental tissue using AllPrep DNA/RNA/Protein Mini Kit from Qiagen.

Paper title
The human placental proteome is affected by maternal smoking
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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA/Protein Mini Kit below.

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