CelLytic™ MT Cell Lysis Reagent
Protein isolation Tissue - Human umbilical cord tissue
Publication protocol
Snap-frozen UCT was segmented into pieces weighing approximately 30 mg and thawed in a 200 ul solution of tissue lysis buffer (Sigma, #C3228) with 5 ul/ml protease inhibitor (Sigma, #P8340). Thawed samples were homogenized and spun at 16,000xg for 10 minutes. The supernatant was collected and stored at −80°C until protein analysis.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
When should I be harvesting the tissue before using it for the most optimal results?
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
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Manufacturer protocol
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