CelLytic™ MT Cell Lysis Reagent

Protein isolation Tissue - Rat skin tissue

Experiment
Protein isolation Tissue - Rat skin tissue
Product
CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- Proteins are extracted in CelLytic™ MT Cell Lysis Reagent mixed with Protease Inhibitor Cocktail P8340 (Sigma, MO) using Mini-Beadbeater-1 (Biospec, OK) with 5.5 g/cc density zirconia beads.

Publication protocol

Tissue was harvested at 2 and 5 weeks after PEF treatment, immediately flash frozen in liquid nitrogen and kept at −80 °C. The center of the treated area was excised and proteins were extracted in CelLytic™ MT Cell Lysis Reagent, C3228 (Sigma, MO) mixed with Protease Inhibitor Cocktail P8340 (Sigma, MO) using Mini-Beadbeater-1 (Biospec, OK) with 5.5 g/cc density zirconia beads (Biospec, OK). Tubes with the buffer and beads were shakes 4 times for 15 seconds with 1-minute intervals, when the tubes were kept on ice. Immediately after the extraction, total protein was quantified using 660 nm Protein Assay (Pierce,IL).

Full paper   Login or join for free to view the full paper.

Reviews

CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Rat skin tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich.

Paper title
Skin Rejuvenation with Non-Invasive Pulsed Electric Fields
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Tissue - Rat skin tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms