CelLytic™ MT Cell Lysis Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Proteins are extracted in CelLytic™ MT Cell Lysis Reagent mixed with Protease Inhibitor Cocktail P8340 (Sigma, MO) using Mini-Beadbeater-1 (Biospec, OK) with 5.5 g/cc density zirconia beads.

Protocol tips
- Proteins are extracted in CelLytic™ MT Cell Lysis Reagent mixed with Protease Inhibitor Cocktail P8340 (Sigma, MO) using Mini-Beadbeater-1 (Biospec, OK) with 5.5 g/cc density zirconia beads.

Publication protocol

Tissue was harvested at 2 and 5 weeks after PEF treatment, immediately flash frozen in liquid nitrogen and kept at −80 °C. The center of the treated area was excised and proteins were extracted in CelLytic™ MT Cell Lysis Reagent, C3228 (Sigma, MO) mixed with Protease Inhibitor Cocktail P8340 (Sigma, MO) using Mini-Beadbeater-1 (Biospec, OK) with 5.5 g/cc density zirconia beads (Biospec, OK). Tubes with the buffer and beads were shakes 4 times for 15 seconds with 1-minute intervals, when the tubes were kept on ice. Immediately after the extraction, total protein was quantified using 660 nm Protein Assay (Pierce,IL).

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

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