B-PER™ II Bacterial Protein Extraction Reagent (2X)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Capable of extracting bacterial microcompartments (BMCs).	- The manufacturer protocol was followed.	- Compatible with most downstream workflows such as electrophoresis, affinity purification, immunoprecipitation, protein interaction analysis, crosslinking and protein labeling.

Upstream tips
- Capable of extracting bacterial microcompartments (BMCs).
Protocol tips
- The manufacturer protocol was followed.
Downstream tips
- Compatible with most downstream workflows such as electrophoresis, affinity purification, immunoprecipitation, protein interaction analysis, crosslinking and protein labeling.

Publication protocol

In order to characterize and compare the structural and biochemical properties of wtBMCs, rBMCs and eBMCs, a number of purification methods to isolate intact BMCs were investigated involving the use of mild detergent lysis procedures, employing either a bacterial specific lysis reagent (B-PER™) or a yeast specific lysis reagent (Y-PER™). The bacterial protein extraction (B-PER™) method is based on lysing cells with B-PER™ and entails consecutive centrifugation steps to separate cell debris from Pdu metabolosomes. This procedure was found to work best for the isolation of complete BMCs (wtBMCs and rBMCs). The yeast protein extraction reagent (Y-PER™) method is based on lysis of bacterial cells and subsequent salt precipitation and was found to be effective for the purification of eBMCs. The purification and isolation of the various BMCs was followed by SDS-PAGE.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for B-PER™ II Bacterial Protein Extraction Reagent (2X) below.

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