CelLytic™ B Plus Kit
Protein isolation Bacteria - Escherichia coli
Publication protocol
Cells were grown for 21 hours, reaching similar densities, harvested and lysed with CelLytic™ B Plus Kit (Sigma-Aldrich).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Hollie Fowler
How can I deal with my pellet being too viscous?
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
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Manufacturer protocol
Download the product protocol from Sigma-Aldrich for CelLytic™ B Plus Kit below.
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