TRI Reagent™ Solution

Protein isolation Bacteria - Vibrio cholerae

Experiment

Protein isolation Bacteria - Vibrio cholerae

Product

TRI Reagent™ Solution from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Protocol tips

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	- The protein is precipitated by pre-chilled acetone at ice for 15 minutes.

Protocol tips
- The protein is precipitated by pre-chilled acetone at ice for 15 minutes.

Publication protocol

Protein extraction using TRI-Reagent kit (Molecular Research Center, Cincinnati, US) was done as recommended by the supplier. Briefly, 1mL of TRI-reagent and chloroform was added sequentially per 5x105 of cells / 0.05g of pus and centrifuged at 12 000g at 4oC for 15 minutes to separate into DNA, RNA and protein layers. Instead of following the manufacturer’s protocol, we modified the protein precipitation method where the protein was precipitated by pre-chilled acetone at ice for 15 minutes.

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Discussion

5 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Bacteria - Vibrio cholerae using TRI Reagent™ Solution from Thermo Fisher Scientific.

Paper title

Optimal protein extraction methods from diverse sample types for protein profiling by using Two-Dimensional Electrophoresis (2DE).

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Manufacturer protocol

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