Qproteome Bacterial Protein Prep Kit

Protein isolation Bacteria - Staphylococcus aureus

Experiment

Protein isolation Bacteria - Staphylococcus aureus

Product

Qproteome Bacterial Protein Prep Kit from Qiagen

Manufacturer

Qiagen

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Protocol tips

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	- Proteins were extracted according to the manufacturer's protocol.

Protocol tips
- Proteins were extracted according to the manufacturer's protocol.

Publication protocol

Briefly, soluble bacterial proteins were extracted from cultures of Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus incubated at 27 °C for 24 h and proteins were obtained using the Qproteome Bacterial Protein Prep Kit (Qiagen, MD, USA) and total DON quantified using a Shimadzu TNM-1(Shimadzu Corporation, Columbia, MD).

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Discussion

5 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

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Papers

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Manufacturer protocol

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