Qproteome Bacterial Protein Prep Kit
Protein isolation Bacteria - Pseudomonas aeruginosa
Publication protocol
Briefly, soluble bacterial proteins were extracted from cultures of Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus incubated at 27 °C for 24 h and proteins were obtained using the Qproteome Bacterial Protein Prep Kit (Qiagen, MD, USA) and total DON quantified using a Shimadzu TNM-1(Shimadzu Corporation, Columbia, MD).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Hollie Fowler
How can I deal with my pellet being too viscous?
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
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