CelLytic™ B Cell Lysis Reagent

Protein isolation Bacteria - Pseudomonas aeruginosa

Experiment

Protein isolation Bacteria - Pseudomonas aeruginosa

Product

CelLytic™ B Cell Lysis Reagent from Sigma-Aldrich

Manufacturer

Sigma-Aldrich

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Protocol tips

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	- Proteins were extracted according to the manufacturer's protocol.

Protocol tips
- Proteins were extracted according to the manufacturer's protocol.

Publication protocol

Overnight cultures of wild-type P. aeruginosa and its derivatives were diluted 100-fold in LB broth and incubated at 37°C until the OD600 reached about 1.5. Total soluble proteins were prepared by using CelLytic B cell lysis reagent (Sigma).

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Discussion

4 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Bacteria - Pseudomonas aeruginosa using CelLytic™ B Cell Lysis Reagent from Sigma-Aldrich.

Paper title

Modulation of Biofilm Dispersal by a Cyclic-Di-GMP Phosphodiesterase with a Putative Hypoxia-Sensing Domain

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ B Cell Lysis Reagent below.

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Videos

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