CelLytic™ B Cell Lysis Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Bacteria are centrifuged and pellets are suspended in 1 ml of 1X CelLytic™ B cell lysis reagent (Sigma), containing 0.2 mg/ml lysozyme (RPI Corp.), 50 U/ml Benzonase® Nuclease (Sigma), and 25 μl of Protease Inhibitor Cocktail (Sigma).

Protocol tips
- Bacteria are centrifuged and pellets are suspended in 1 ml of 1X CelLytic™ B cell lysis reagent (Sigma), containing 0.2 mg/ml lysozyme (RPI Corp.), 50 U/ml Benzonase® Nuclease (Sigma), and 25 μl of Protease Inhibitor Cocktail (Sigma).

Publication protocol

Wild type and ST-2W bacteria were grown overnight in LB Broth. Bacteria were centrifuged and pellets were suspended in 1 ml of 1X CelLytic™ B cell lysis reagent (Sigma), containing 0.2 mg/ml lysozyme (RPI Corp.), 50 U/ml Benzonase® Nuclease (Sigma), and 25 μl of Protease Inhibitor Cocktail (Sigma). Cell extracts were centrifuged and supernatants containing soluble proteins were removed for SDS-PAGE analysis.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ B Cell Lysis Reagent below.

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