Qproteome Bacterial Protein Prep Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- For soluble protein preparations from up to 4.5 liters of bacterial cell culture. - Before use, Native Lysis Buffer must be supplemented with lysozyme and Benzonase® Nuclease.		- Downstream applications are SDS-PAGE and mass spectrometry. - Solubilization of inclusion bodies requires high concentrations of denaturants (e.g., 6M Guanidine HCl).

Upstream tips
- For soluble protein preparations from up to 4.5 liters of bacterial cell culture. - Before use, Native Lysis Buffer must be supplemented with lysozyme and Benzonase® Nuclease.
Downstream tips
- Downstream applications are SDS-PAGE and mass spectrometry. - Solubilization of inclusion bodies requires high concentrations of denaturants (e.g., 6M Guanidine HCl).

Publication protocol

The cultures were grown until mid-exponential phase (OD of 2.7), and the 50 ml volume of cultures were used for a total protein extraction by using Qproteome Bacterial Protein Prep kit (Qiagen). Disulfide bonds within proteins were reduced with 2-Mercaptoethanol and separated by SDS-PAGE gel electrophoresis.

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Manufacturer protocol

Download the product protocol from Qiagen for Qproteome Bacterial Protein Prep Kit below.

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