CelLytic™ B Cell Lysis Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Before adding CelLytic B reagent resuspend bacterial pellets in 1.0 ml of 50 mM Tris-HCl, pH 9.5 (three times). - The pellets are lysed by shaking at 37°C for 30 minutes in 1.0 ml of CelLytic B reagent in 50 mM Tris-HCl, pH 9.5. - The pellets are recovered by centrifugation and resuspended in 1.0 ml of 50 mM Tris-HCl, pH 9.5 (three times). Finally the pellets are resuspended in 1.0 ml of 50 mM Tris-HCl, pH 9.5.

Protocol tips

- Before adding CelLytic B reagent resuspend bacterial pellets in 1.0 ml of 50 mM Tris-HCl, pH 9.5 (three times). - The pellets are lysed by shaking at 37°C for 30 minutes in 1.0 ml of CelLytic B reagent in 50 mM Tris-HCl, pH 9.5. - The pellets are recovered by centrifugation and resuspended in 1.0 ml of 50 mM Tris-HCl, pH 9.5 (three times). Finally the pellets are resuspended in 1.0 ml of 50 mM Tris-HCl, pH 9.5.

Publication protocol

Cultures were harvested at 24 hours by centrifugation. Pellets were resuspended in 1.0 ml of 50 mM Tris-HCl, pH 9.5 and pelleted; this was repeated three times. The pellets were then lysed by shaking at 37°C for 30 minutes in 1.0 ml of CelLytic B reagent in 50 mM Tris-HCl, pH 9.5. The pellets containing the cell walls were recovered by centrifugation and resuspended in 1.0 ml of 50 mM Tris-HCl, pH 9.5 and pelleted; this was repeated three times. Finally the pellets were resuspended in 1.0 ml of 50 mM Tris-HCl, pH 9.5.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ B Cell Lysis Reagent below.

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