|- Pelleted cells are resuspended in 4 mL of CelLyticTM B containing 10 μg/mL (w/v) lysozyme and shaken on a vortexer for 30 min. After, samples are centrifuged (10,000g, RT, 5 min).|
CelLytic™ B Cell Lysis Reagent from Sigma-Aldrich has not yet been reviewed for this experiment
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2 years ago
Author: Hollie Fowler
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
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