Y-PER™ Yeast Protein Extraction Reagent

Protocol tips

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	- The cells are harvested at late exponential phase and the total protein is extracted using Y-PER extraction solution according to manufacturer’s instructions.

Protocol tips
- The cells are harvested at late exponential phase and the total protein is extracted using Y-PER extraction solution according to manufacturer’s instructions.

Publication protocol

Overnight cultures were inoculated in 250 mL shake flasks containing 25 mL YNB supplemented with d-glucose (20 g L−1) at a starting optical density of 0.25 at 620 nm (Ultrospec 2100 Pro spectrophotometer, Amersham Biosciences Corp., USA) and grown at 30 °C and 180 rpm. The cells were harvested at late exponential phase and the total protein was extracted using Y-PER extraction solution (Pierce, Illinois, USA) according to manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Y-PER™ Yeast Protein Extraction Reagent below.

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