Y-PER™ Yeast Protein Extraction Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- After the incubation periods, cells are pelleted at 3000 x g for 5 minutes at 4°C. The pellets are suspended in 250 μL Y-PER reagent and vortexed gently at room temperature for 20 minutes. The mixture is centrifuged at 14,000 x g for 10 minutes saving the supernatant.

Protocol tips
- After the incubation periods, cells are pelleted at 3000 x g for 5 minutes at 4°C. The pellets are suspended in 250 μL Y-PER reagent and vortexed gently at room temperature for 20 minutes. The mixture is centrifuged at 14,000 x g for 10 minutes saving the supernatant.

Publication protocol

To exclude significant loss in cell mass, protein was extracted using Y-PER Yeast Protein Extraction Reagent (Thermo Scientific). Yeast numbers, germination conditions and timing were the same as those for the RNA extraction and XTT experiments. After the incubation periods, cells were pelleted at 3000 x g for 5 minutes at 4°C. The pellets were suspended in 250 μL Y-PER reagent and vortexed gently at room temperature for 20 minutes. The mixture was centrifuged at 14,000 x g for 10 minutes saving the supernatant containing the soluble protein. The protein concentration was measured with the Qubit 2.0 fluorometer using the Qubit Protein Assay Kit (Life Technologies).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Y-PER™ Yeast Protein Extraction Reagent below.

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