Y-PER™ Yeast Protein Extraction Reagent

Protocol tips

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	- The extraction was performed according to the manufacturer's protocol.

Protocol tips
- The extraction was performed according to the manufacturer's protocol.

Publication protocol

The enzymatic activity of xylose reductase (XR) and xylitol dehydrogenase (XDH) in crude-cell extracts was measured according to [23]. For this, 5 mL of cell suspension of S. stipitis, S. passalidarum, S. arborariae and C. tenuis were collected in the middle of the exponential growth phase during aerobic and oxygen-limited fermentations. Cells were pelleted by centrifugation, washed with sterile water, and lysed with Y-PER®—Yeast Protein Extraction Reagent (Pierce, Rockford, USA) to obtain cell-crude extracts. Protein concentrations in cell-free extracts were determined using Quick StartTM Bradford Protein Assay Kit (Bio-Rad Laboratories Ltda., USA), following the manufacture’s instruction.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Y-PER™ Yeast Protein Extraction Reagent below.

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