CelLytic™ Y Plus Kit

Protocol tips

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	- Extraction was performed according to the manufacturer's protocol.

Protocol tips
- Extraction was performed according to the manufacturer's protocol.

Publication protocol

The pyruvate decarboxylase activity of strains No. 2201 and KY2132 was measured as described in Flikweert et al. (1996). Cell extracts were prepared from yeast cells, incubated in 5 ml YPD10 to late log phase at 30 °C, using CelLytic Y Cell Lysis Reagent (Sigma). Pyruvate decarboxylase activity was assayed at 30 °C immediately after extract preparation, using a Molecular Devices VersaMax microplate reader set at 340 nm. Protein concentrations in cell‐free extracts were determined using Protein Assay CBB Solution (Nacalai Tesque) with bovine serum albumin (Sigma) as a standard.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ Y Plus Kit below.

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