pcDNA™3.1 (+) Mammalian Expression Vector

Protein expression and purification Mammalian cells - CHO-K1 sRAGE

Experiment
Protein expression and purification Mammalian cells - CHO-K1 sRAGE
Product
pcDNA™3.1 (+) Mammalian Expression Vector from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

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Discussion

Discussion

5 years ago

Author: Anders Bergman Sweden

About expressing a trimeric protein complex from mammalian tissue

I am planning on doing an analysis via crystallization on a trimeric protein complex from mammalian tissue culture cells. Is it possible to express and purify such a protein from mammalian tissue?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein expression and purification Mammalian cells - CHO-K1 sRAGE using pcDNA™3.1 (+) Mammalian Expression Vector from Thermo Fisher Scientific.

Paper title
GeneOptimizer Program-assisted cDNA Reengineering Enhances sRAGE Autologous Expression in Chinese Hamster Ovary Cells
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for pcDNA™3.1 (+) Mammalian Expression Vector below.

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