We haven't found the publication's protocol yet.
Champion™ pET SUMO Expression System from Thermo Fisher Scientific has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
4 years ago
I am currently working with E.coli and I am trying to express thermophilic genes. What temperature should I be using for incubation after IPTG induction? I have read some papers that suggested to incubate the cells at 42 degrees for a short amount of time. Is this going to affect the protein?
4 years ago
Hi everyone! Currently I am attempting to measure the beta -galactosidase activity by inducing the lac operon in E.coli cells. I am trying to grow my cells in alkaline conditions with a pH > 8.5 but when I induce the cells with IPTG they are not growing. The IPTG concentration I have tried using is 0.1 and 1mM. Is there a chance that IPTG in these concentrations in alkaline conditions becomes toxic to my bacteria? Should I lower the concentration or do you think that the problem lies elsewhere?
Check out relevant papers found by Labettor's AI that are relevant for performing Protein expression and purification Bacteria - Escherichia coli IFNA2 using Champion™ pET SUMO Expression System from Thermo Fisher Scientific.
Download the product protocol from Thermo Fisher Scientific for Champion™ pET SUMO Expression System below.
We haven't found the manufacturer protocol for this product yet.
Check out videos that might be relevant for performing Protein expression and purification Bacteria - Escherichia coli IFNA2 using Champion™ pET SUMO Expression System from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Fill out your contact details and receive price quotes in your InboxOutsource experiment