pQE-30 Xa Vector
Protein expression and purification Bacteria - Escherichia coli Siva1
Protocol tips
Publication protocol
We haven't found the publication's protocol yet.
Reviews
pQE-30 Xa Vector from Qiagen has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Discussion
5 years ago
Author:
What temperature should I use for expressing thermophillic genes in E. coli?
I am currently working with E.coli and I am trying to express thermophilic genes. What temperature should I be using for incubation after IPTG induction? I have read some papers that suggested to incubate the cells at 42 degrees for a short amount of time. Is this going to affect the protein?
Discussion
5 years ago
Author:
Can IPTG in alkaline conditions become toxic to the bacteria?
Hi everyone! Currently I am attempting to measure the beta -galactosidase activity by inducing the lac operon in E.coli cells. I am trying to grow my cells in alkaline conditions with a pH > 8.5 but when I induce the cells with IPTG they are not growing. The IPTG concentration I have tried using is 0.1 and 1mM. Is there a chance that IPTG in these concentrations in alkaline conditions becomes toxic to my bacteria? Should I lower the concentration or do you think that the problem lies elsewhere?
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Protein expression and purification Bacteria - Escherichia coli Siva1 using pQE-30 Xa Vector from Qiagen.
Manufacturer protocol
Download the product protocol from Qiagen for pQE-30 Xa Vector below.
We haven't found the manufacturer protocol for this product yet.
Videos
Check out videos that might be relevant for performing Protein expression and purification Bacteria - Escherichia coli Siva1 using pQE-30 Xa Vector from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Outsource your experiment
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment