Wizard® Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram negative Legionella

Experiment

DNA isolation / purification Bacteria - Gram negative Legionella

Product

Wizard® Genomic DNA Purification Kit from Promega

Manufacturer

Promega

Buy product Write review

Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		If the DNA pellet is difficult to dissolve, Rehydrate DNA by incubating 1 hour at 65°C, and then leave the sample at room temperature or 4°C overnigh

Downstream tips
If the DNA pellet is difficult to dissolve, Rehydrate DNA by incubating 1 hour at 65°C, and then leave the sample at room temperature or 4°C overnigh

Publication protocol

DNA Extraction from spp. for PCR amplification.
DNA extraction was performed as previously explained (). Conditions of PCR and size of the amplified fragments were as described by Hosseini et al. (). DNA of ATCC 33152 was used as the positive control. PCR products were analyzed by agarose gel electrophoresis using 1.5% agarose gel.

Full paper Login or join for free to view the full paper.

Reviews

Wizard® Genomic DNA Purification Kit from Promega has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Comment View 2 comments

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Comment View 1 comment

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Legionella using Wizard® Genomic DNA Purification Kit from Promega.

Paper title

Microbiological qualification of air, water and dialysate in a haemodialysis centre: a new focus on spp.

Full paper

Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Bacteria - Gram negative Legionella using Wizard® Genomic DNA Purification Kit from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment