Wizard® Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram negative Legionella

Experiment
DNA isolation / purification Bacteria - Gram negative Legionella
Product
Wizard® Genomic DNA Purification Kit from Promega
Manufacturer
Promega

Protocol tips

Downstream tips
If the DNA pellet is difficult to dissolve, Rehydrate DNA by incubating 1 hour at 65°C, and then leave the sample at room temperature or 4°C overnigh

Publication protocol

DNA Extraction from spp. for PCR amplification.
DNA extraction was performed as previously explained (). Conditions of PCR and size of the amplified fragments were as described by Hosseini et al. (). DNA of ATCC 33152 was used as the positive control. PCR products were analyzed by agarose gel electrophoresis using 1.5% agarose gel.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Legionella using Wizard® Genomic DNA Purification Kit from Promega.

Paper title
Microbiological qualification of air, water and dialysate in a haemodialysis centre: a new focus on spp.
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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

Download PDF Download manufacturer protocol

Videos

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