pCEP4 Mammalian Expression Vector

Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4

Experiment
Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4
Product
pCEP4 Mammalian Expression Vector from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Publication protocol

We haven't found the publication's protocol yet.

Reviews

pCEP4 Mammalian Expression Vector from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Poland

What temperature should I use for expressing thermophillic genes in E. coli?

I am currently working with E.coli and I am trying to express thermophilic genes. What temperature should I be using for incubation after IPTG induction? I have read some papers that suggested to incubate the cells at 42 degrees for a short amount of time. Is this going to affect the protein?

Discussion

5 years ago

Author: Finland

Can IPTG in alkaline conditions become toxic to the bacteria?

Hi everyone! Currently I am attempting to measure the beta -galactosidase activity by inducing the lac operon in E.coli cells. I am trying to grow my cells in alkaline conditions with a pH > 8.5 but when I induce the cells with IPTG they are not growing. The IPTG concentration I have tried using is 0.1 and 1mM. Is there a chance that IPTG in these concentrations in alkaline conditions becomes toxic to my bacteria? Should I lower the concentration or do you think that the problem lies elsewhere?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4 using pCEP4 Mammalian Expression Vector from Thermo Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for pCEP4 Mammalian Expression Vector below.

We haven't found the manufacturer protocol for this product yet.

Videos

Check out videos that might be relevant for performing Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4 using pCEP4 Mammalian Expression Vector from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms