Pierce™ Cell Surface Protein Isolation Kit

Protein isolation Tissue - Human aortic endothelial cells

Experiment
Protein isolation Tissue - Human aortic endothelial cells
Product
Pierce™ Cell Surface Protein Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
To extract membrane proteins and cell surface proteins, cell lysates were prepared and processed from proliferating (PDL23) and senescent (PDL59) WI-38 fibroblasts using the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Scientific) or the Pierce™ Cell Surface Protein Isolation Kit (Thermo Scientific).

Publication protocol

To extract membrane proteins and cell surface proteins, cell lysates were prepared and processed from proliferating (PDL23) and senescent (PDL59) WI-38 fibroblasts using the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Scientific) or the Pierce™ Cell Surface Protein Isolation Kit (Thermo Scientific).

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Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Human aortic endothelial cells using Pierce™ Cell Surface Protein Isolation Kit from Thermo Fisher Scientific.

Paper title
SCAMP4 enhances the senescent cell secretome
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Pierce™ Cell Surface Protein Isolation Kit below.

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