MethylFlash™ Methylated DNA Quantification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	gDNA was extracted using the NucleoSpin® Tissue kit (740952, Macherey Nagel). Global DNA methylation was quantified using the MethylFlash methylated DNA quantification kit (P-1034, Epigentek, France). Methyl DNA collection were performed using the “Methyl-Collector Ultra kit” (55005, Active Motif). Histone 3 acetylation was quantified using the EpiQuik Tissue Acetyl-Histone H3 ChIP Kit (P-2012, Epigentek). ChIP was performed using the ChIP-IT High Sensitivity kit (53040, Active Motif) with ChIP grade antibodies (Table ). All these kits were used according to the manufacturer’s instructions.

Protocol tips

gDNA was extracted using the NucleoSpin® Tissue kit (740952, Macherey Nagel). Global DNA methylation was quantified using the MethylFlash methylated DNA quantification kit (P-1034, Epigentek, France). Methyl DNA collection were performed using the “Methyl-Collector Ultra kit” (55005, Active Motif). Histone 3 acetylation was quantified using the EpiQuik Tissue Acetyl-Histone H3 ChIP Kit (P-2012, Epigentek). ChIP was performed using the ChIP-IT High Sensitivity kit (53040, Active Motif) with ChIP grade antibodies (Table ). All these kits were used according to the manufacturer’s instructions.

Publication protocol

Epigenetics
gDNA was extracted using the NucleoSpin® Tissue kit (740952, Macherey Nagel). Global DNA methylation was quantified using the MethylFlash methylated DNA quantification kit (P-1034, Epigentek, France). Methyl DNA collection were performed using the “Methyl-Collector Ultra kit” (55005, Active Motif). Histone 3 acetylation was quantified using the EpiQuik Tissue Acetyl-Histone H3 ChIP Kit (P-2012, Epigentek). ChIP was performed using the ChIP-IT High Sensitivity kit (53040, Active Motif) with ChIP grade antibodies (Table ). All these kits were used according to the manufacturer’s instructions. Primers used in this study were designed with the primer3 software []: (F: 5′-AAAGCCAACCGTCTTTGCTA-3′ and R: 5′-GCCACTTCCCTATTCACCAA-3′), (MC1 F: 5′-GTCAGGCTGGTCTCGAACTC-3′ and R: 5′-CGCTCCTGAACAGCAACATA-3′) and (MC2 F: 5′-AAGGAAACGCAGTGAGACAGA-3′ and R: 5′-AGCTGGGAGCACAAAAACAG-3′), (F: 5′AATTCCCCAGACTTCCCCTA-3′ and R: 5′-GGTGGCGAAGAAGTTGGTTA-3′).



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Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Whole genome profiling - MCF-7, MDA-MB-453 human breast cancer using MethylFlash™ Methylated DNA Quantification Kit from Epigentek.

Manufacturer protocol

Download the product protocol from Epigentek for MethylFlash™ Methylated DNA Quantification Kit below.

Download manufacturer protocol

Videos

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