RNeasy Mini Kit

RNA isolation / purification Tissue - human adipose tissue

Experiment
RNA isolation / purification Tissue - human adipose tissue
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

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Publication protocol

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - human adipose tissue using RNeasy Mini Kit from Qiagen.

Paper title
Determinants of Human Adipose Tissue Gene Expression: Impact of Diet, Sex, Metabolic Status, and Genetic Regulation
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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Videos

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