DNeasy Blood & Tissue Kit

DNA isolation / purification Bacteria - Gram positive piezophilic bacteria [AT7 and AT12 Strains]

Experiment
DNA isolation / purification Bacteria - Gram positive piezophilic bacteria [AT7 and AT12 Strains]
Product
DNeasy Blood & Tissue Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

16S characterization of isolates.
Genomic DNA was extracted from pure cultures using a DNeasy kit (QIAGEN, Valencia, CA). PCR amplification was performed using the general bacterial primers 27F and 1492R (17). Automated DNA sequencing was performed on an Amersham MEGABace 500 system (Amersham Pharmacia Biotech) using general eubacterial primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′), 518R (5′-GTATTACCGCGGCTGCTG-3′), 530F (5′-GTGCCAGCAGCCGCGG-3′), 907R (5′-CCGTCAATTCATTTGAGT-3′), 926F (5′-ACTCAAAGGAATTGACGG-3′), and 1492R (5′-GGTTACCTTGTTACGACTT-3′). The resulting chromatograms were assembled into contigs by use of Sequencher software (Gene Codes Corp., Ann Arbor, MI).

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive piezophilic bacteria [AT7 and AT12 Strains] using DNeasy Blood & Tissue Kit from Qiagen.

Paper title
The Unique 16S rRNA Genes of Piezophiles Reflect both Phylogeny and Adaptation
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download PDF Download manufacturer protocol

Videos

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