DNeasy Blood & Tissue Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

16S characterization of isolates.
Genomic DNA was extracted from pure cultures using a DNeasy kit (QIAGEN, Valencia, CA). PCR amplification was performed using the general bacterial primers 27F and 1492R (17). Automated DNA sequencing was performed on an Amersham MEGABace 500 system (Amersham Pharmacia Biotech) using general eubacterial primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′), 518R (5′-GTATTACCGCGGCTGCTG-3′), 530F (5′-GTGCCAGCAGCCGCGG-3′), 907R (5′-CCGTCAATTCATTTGAGT-3′), 926F (5′-ACTCAAAGGAATTGACGG-3′), and 1492R (5′-GGTTACCTTGTTACGACTT-3′). The resulting chromatograms were assembled into contigs by use of Sequencher software (Gene Codes Corp., Ann Arbor, MI).

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download manufacturer protocol

Videos

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