GeneJET Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp

Experiment
DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp
Product
GeneJET Genomic DNA Purification Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Close the bag with GeneJET Genomic DNA Purification Columns tightly
after each use
Downstream tips
If residual solution is seen in the purification column after washing the
column with Wash Buffer II, empty the collection tube and re-spin the
column for an additional 1 min. at maximum speed (≥12000  g).

Publication protocol

Genomic DNA of the bacterial isolates was extracted using the Gene JET Genomic DNA purification kit (Thermo Scientific # k0721). The 16s rRNA fragments were partially amplified by PCR using Maxima Hot Start PCR Master Mix (Thermo K1051) in Sigma Company of Scientific Services, Egypt (www.sigma-co-eg.com). A region of ∼1000 bp from the 16s rRNA gene was amplified using the primers: forward primer AGA GTT TGA TCC TGG CTC AG and the reverse primer GGT TAC CTT GTT ACG ACT T. The PCR program consisted of one cycle of DNA initial denaturation at 95°C (10 min), 35 cycles of 95°C (30 s), 65°C (1 min), and 72°C (1 min and 30 s), plus one additional cycle of a final chain elongation at 72°C (10 min). Finally sequencing was accomplished to the PCR product on GATC Company by use of an ABI 3730xl DNA sequencer by using forward and reverse primers.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for GeneJET Genomic DNA Purification Kit below.

Download PDF Download manufacturer protocol

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