GeneJET Genomic DNA Purification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Close the bag with GeneJET Genomic DNA Purification Columns tightly after each use	If residual solution is seen in the purification column after washing the column with Wash Buffer II, empty the collection tube and re-spin the column for an additional 1 min. at maximum speed (≥12000  g).

Protocol tips
Close the bag with GeneJET Genomic DNA Purification Columns tightly after each use
Downstream tips
If residual solution is seen in the purification column after washing the column with Wash Buffer II, empty the collection tube and re-spin the column for an additional 1 min. at maximum speed (≥12000  g).

Publication protocol

Genomic DNA of the bacterial isolates was extracted using the Gene JET Genomic DNA purification kit (Thermo Scientific # k0721). The 16s rRNA fragments were partially amplified by PCR using Maxima Hot Start PCR Master Mix (Thermo K1051) in Sigma Company of Scientific Services, Egypt (www.sigma-co-eg.com). A region of ∼1000 bp from the 16s rRNA gene was amplified using the primers: forward primer AGA GTT TGA TCC TGG CTC AG and the reverse primer GGT TAC CTT GTT ACG ACT T. The PCR program consisted of one cycle of DNA initial denaturation at 95°C (10 min), 35 cycles of 95°C (30 s), 65°C (1 min), and 72°C (1 min and 30 s), plus one additional cycle of a final chain elongation at 72°C (10 min). Finally sequencing was accomplished to the PCR product on GATC Company by use of an ABI 3730xl DNA sequencer by using forward and reverse primers.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for GeneJET Genomic DNA Purification Kit below.

Download manufacturer protocol

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