High Pure FFPET RNA Isolation Kit

RNA isolation / purification Tissue - human breast tissue

Experiment
RNA isolation / purification Tissue - human breast tissue
Product
High Pure FFPET RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Publication protocol

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - human breast tissue using High Pure FFPET RNA Isolation Kit from Roche Lifesciences.

Paper title
Accurate RNA Sequencing From Formalin-Fixed Cancer Tissue To Represent High-Quality Transcriptome From Frozen Tissue
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure FFPET RNA Isolation Kit below.

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Videos

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