Gentra Puregene Blood Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not incubate samples with lysis buffer overnight at 37°C. 2-3 hrs is enough

Protocol tips
Do not incubate samples with lysis buffer overnight at 37°C. 2-3 hrs is enough

Publication protocol

DNA extraction and freeze drying
Cells were bulk cultured (ECACC, Porton Down, UK) to approximately 1010 cells and frozen as pellets of 108 cells. Genomic DNA was extracted from cell pellets using the PureGene DNA purification and Autopure LS equipment (Qiagen, Crawley, UK). The integrity of the extracted DNA was confirmed by electrophoresis in agarose gels. Each of the DNA samples was dissolved at a concentration of approximately 29 μg/ml in 0.8 m Tris/0.08 m EDTA buffer with 2.5 mg/ml Trehalose. A volume of 0.8 ml of this solution was dispensed into a minimum of 1200 glass ampoules and freeze-dried. Sealed ampoules were stored at −20 °C.

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Manufacturer protocol

Download the product protocol from Qiagen for Gentra Puregene Blood Kit below.

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