Gentra Puregene Blood Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Frozen samples should be thawed and equilibrated to room temperature (15–25°C) before beginning the procedure.

Upstream tips
Frozen samples should be thawed and equilibrated to room temperature (15–25°C) before beginning the procedure.

Publication protocol

Genomic DNA and RNA isolation.
Magnetic bead depletion was performed on PBMCs to enrich for resting CD4 T cells as described previously (). Genomic DNA was purified from resting CD4 T cells using the Puregene kit (Gentra). 10–20 ml of plasma from each patient was ultracentrifuged at 25,200 for 2 h at 4° to concentrate plasma virions. Viral RNA was isolated from pelleted virions using the QIAGEN Viral RNA isolation kit (QIAGEN). In brief, supernatant was removed after centrifugation, and pelleted virions were resuspended in the supplied lysis buffer. RNA was isolated according to the recommended protocol.

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Manufacturer protocol

Download the product protocol from Qiagen for Gentra Puregene Blood Kit below.

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