TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

The SHFVic LVR strain was constructed from cDNA amplified from the viral genome RNA as described previously. SHFV RNA (100 ng) transcribed from the infectious clone in vitro was transfected into MA104 cells, and culture fluid containing progeny virus was collected at 120 h after transfection. The progeny virus was passaged once by infecting MA104 cells at an MOI of 1, and the culture fluid was harvested at 24 hpi, clarified by centrifugation, aliquoted, and stored at –80 °C. The titer of the SHFVic stock was 1.3 × 107 pfu/mL.

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Manufacturer protocol

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