TRIzol Reagent

RNA isolation / purification Cells - immortalized MA-104

Experiment
RNA isolation / purification Cells - immortalized MA-104
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

The SHFVic LVR strain was constructed from cDNA amplified from the viral genome RNA as described previously. SHFV RNA (100 ng) transcribed from the infectious clone in vitro was transfected into MA104 cells, and culture fluid containing progeny virus was collected at 120 h after transfection. The progeny virus was passaged once by infecting MA104 cells at an MOI of 1, and the culture fluid was harvested at 24 hpi, clarified by centrifugation, aliquoted, and stored at –80 °C. The titer of the SHFVic stock was 1.3 × 107 pfu/mL.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized MA-104 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Rotavirus infection induces G1 to S phase transition in MA104 cells via Ca⁺²/Calmodulin pathway.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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