Gentra Puregene Yeast/Bact. Kit

DNA isolation / purification Yeast - Candida albicans

Experiment
DNA isolation / purification Yeast - Candida albicans
Product
Gentra Puregene Yeast/Bact. Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
Frozen yeast/bacterial samples should be thawed and equilibrated to room temperature (15–25°C) before beginning the procedure.

Publication protocol

Protocol A: Using QIAamp DNA mini kit, with prior chemical treatment. In this protocol, we introduced a chemical treatment with sodium dodecyl sulphate and/or β-mercaptoethanol, before commercial kit extraction step.

Protocol B: QIAamp DNA mini kit with glass beads. Extraction was performed using a modification of protocol A, where the chemical treatment step was replaced with boiling for 10 min followed by freezing the sample at −50°C and agitation with 0.5 mm glass beads. Qiagen buffer ATL was added to each tube and a volume of sterile glass beads approximately equal to 100 µl was added. Samples were processed for 30 s pulse at 5000 rpm in a Mini-BeadBeater, followed by centrifugation at 13,200 rpm in a centrifuge for 10 min.

Protocol C: This protocol used completely automated QIAcube extraction using QIAamp DNA mini kit. For this protocol, the initial concentration of the sample was fixed 0.5–1.0 McFarland standard.

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Discussion

Discussion

2 years ago

Author: France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Candida albicans using Gentra Puregene Yeast/Bact. Kit from Qiagen.

Paper title
Monochrome LightCycler PCR assay for detection and quantification of five common species of Candida and Aspergillus.
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Manufacturer protocol

Download the product protocol from Qiagen for Gentra Puregene Yeast/Bact. Kit below.

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Videos

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