Gentra Puregene Yeast/Bact. Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Frozen yeast/bacterial samples should be thawed and equilibrated to room temperature (15–25°C) before beginning the procedure.

Upstream tips
Frozen yeast/bacterial samples should be thawed and equilibrated to room temperature (15–25°C) before beginning the procedure.

Publication protocol

Protocol A: Using QIAamp DNA mini kit, with prior chemical treatment. In this protocol, we introduced a chemical treatment with sodium dodecyl sulphate and/or β-mercaptoethanol, before commercial kit extraction step.

Protocol B: QIAamp DNA mini kit with glass beads. Extraction was performed using a modification of protocol A, where the chemical treatment step was replaced with boiling for 10 min followed by freezing the sample at −50°C and agitation with 0.5 mm glass beads. Qiagen buffer ATL was added to each tube and a volume of sterile glass beads approximately equal to 100 µl was added. Samples were processed for 30 s pulse at 5000 rpm in a Mini-BeadBeater, followed by centrifugation at 13,200 rpm in a centrifuge for 10 min.

Protocol C: This protocol used completely automated QIAcube extraction using QIAamp DNA mini kit. For this protocol, the initial concentration of the sample was fixed 0.5–1.0 McFarland standard.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Candida albicans using Gentra Puregene Yeast/Bact. Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for Gentra Puregene Yeast/Bact. Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Yeast - Candida albicans using Gentra Puregene Yeast/Bact. Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.