QIAamp Viral RNA Mini Kit

RNA isolation / purification Cells - immortalized Vero

Experiment
RNA isolation / purification Cells - immortalized Vero
Product
QIAamp Viral RNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- RNA was eluted in 60 μl of AVE buffer and stored at -80°C until use.
Downstream tips
- The concentration and purity of each RNA sample were measured by Thermo Scientific NanoDrop 8000 Spectrophotometer and Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano Kit

Publication protocol

Viral RNA was isolated from 140 μL of each biological specimens and cell culture supernatant using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations. RNA was eluted in 60 μl of AVE buffer and stored at -80°C until use. The concentration and purity of each RNA sample were measured by Thermo Scientific NanoDrop 8000 Spectrophotometer and Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano Kit according the manufacturer’s instructions.

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Vero using QIAamp Viral RNA Mini Kit from Qiagen.

Paper title
Isolation of Infective Zika Virus from Urine and Saliva of Patients in Brazil
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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp Viral RNA Mini Kit below.

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