illustra™ RNAspin Mini Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from up to 5x10^6 cells

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^6 cells

Publication protocol

Genomic DNA extraction from 100 µL of cells in suspension was performed using an Illustra RNAspin Mini RNA Isolation kit (GE Healthcare®) without RNase addition, according to the standard operating procedure (FUNED). Additional DNA samples from Vero cells infected with R. parkeri, R. rhipicephali and Rickettsia amblyommii were obtained using the extraction method described previously, using a QIAamp DNA Blood Mini Kit (Qiagen®) and a High Pure Viral Nucleic Acid Kit (Roche Applied Science®), to test quality by qPCR. DNA samples were quantified using a NanoVue Plus spectrophotometer (GE Healthcare Bio-Sciences AB®), and DNA integrity was analysed by 1% agarose gel electrophoresis

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Manufacturer protocol

Download the product protocol from GE Healthcare Life Sciences for illustra™ RNAspin Mini Isolation Kit below.

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