High Pure RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- To decrease RNase activity,process starting material immeditely or store at 80C. - To increase nucleic yield or purity store all buffer at +15C to +25C.	- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately.	.

Upstream tips
- To decrease RNase activity,process starting material immeditely or store at 80C. - To increase nucleic yield or purity store all buffer at +15C to +25C.
Protocol tips
- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately.
Downstream tips
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Publication protocol

Cell cultures were grown (75–80% confluence), total RNA isolated by using High Pure RNA Isolation kits (Roche Diagnostics, Germany), and RNA concentration determined (absorbance at 260 nm [31]). Transcripts encoding canine estrogen receptor alpha (ER1), progesterone receptor (PR), and epidermal growth factor receptors 1–4 (EGFr/c-erbB-1/HER1, c-erbB-2/HER2, c-erbB-3/HER3, and c-erbB-4/HER4) were amplified by using sequence-specific primers designed based on canine transcript or genomic sequences in Genbank (National Center for Biotechnology Information, USA). Synthesis and amplification of cDNA was performed via QrtPCR by using a Bio-Rad iCycler iQ Multicolor PCR Detection System using an ABsoluteTM QPCR SYBR Green Fluorescein Mix (Thermo Scientific, USA)

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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

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