High Pure RNA Isolation Kit

RNA isolation / purification Cells - immortalized CMT12

Experiment
RNA isolation / purification Cells - immortalized CMT12
Product
High Pure RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
- To decrease RNase activity,process starting material immeditely or store at 80C.

- To increase nucleic yield or purity store all buffer at +15C to +25C.
Protocol tips
- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately.
Downstream tips

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Publication protocol

Cell cultures were grown (75–80% confluence), total RNA isolated by using High Pure RNA Isolation kits (Roche Diagnostics, Germany), and RNA concentration determined (absorbance at 260 nm [31]). Transcripts encoding canine estrogen receptor alpha (ER1), progesterone receptor (PR), and epidermal growth factor receptors 1–4 (EGFr/c-erbB-1/HER1, c-erbB-2/HER2, c-erbB-3/HER3, and c-erbB-4/HER4) were amplified by using sequence-specific primers designed based on canine transcript or genomic sequences in Genbank (National Center for Biotechnology Information, USA). Synthesis and amplification of cDNA was performed via QrtPCR by using a Bio-Rad iCycler iQ Multicolor PCR Detection System using an ABsoluteTM QPCR SYBR Green Fluorescein Mix (Thermo Scientific, USA)

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized CMT12 using High Pure RNA Isolation Kit from Roche Lifesciences.

Paper title
Estrogen receptor-α, progesterone receptor, and c-B/HER-family receptor mRNA detection and phenotype analysis in spontaneous canine models of breast cancer
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

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