TRIzol Reagent

RNA isolation / purification Cells - immortalized D-17

Experiment
RNA isolation / purification Cells - immortalized D-17
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated from cells using Trizol (Invitrogen, Carlsbad, CA), and purified by PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. cDNA was produced from 250ng of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Life Technologies, Carlsbad CA) according to the manufacturer’s protocol. qPCR was performed using TaqMan Gene Expression Master Mix with the canine β-catenin/CTNNB1 (Cf02667771_m1) TaqMan Gene Expression Assay (Applied Biosystems) according to the manufacture’s protocol, on a Bio-Rad iQ5 Multicolor Real-Time PCR Detection System with Bio-Rad iCycler machine and iQ5 software. Ct values were normalized to 18S expression (4352930E, Applied Biosystems). Relative differences in mRNA expression of transfected/treated cells were compared to untreated cells using the ΔΔCt method.20 Gene expression of samples was measured in triplicate.

Full paper   Login or join for free to view the full paper.

Reviews

TRIzol Reagent from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized D-17 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
β-catenin transcriptional activity is minimal in canine osteosarcoma and its targeted inhibition results in minimal changes to cell line behavior
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized D-17 using TRIzol Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms