RNeasy Mini Kit

RNA isolation / purification Cells - immortalized DH82

Experiment
RNA isolation / purification Cells - immortalized DH82
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

The Ehrlichia chaffeensis Wakulla strain (originally provided by the Centers for Disease Control and Prevention, Atlanta, GA) was propagated in a canine macrophage cell line DH82 as described previously24 in DMEM supplemented with 10% FBS and 2% L-glutamine. At 3 d post infection, when ~95% infected of cells were infected, the DH82 cells (~5 × 106 cells) were harvested, and total RNA was extracted using an RNeasy Mini Kit (Qiagen, Valencia, CA, USA) with the optional on column DNase-digestion per the manufacturer’s protocol.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized DH82 using RNeasy Mini Kit from Qiagen.

Paper title
Efficient Enrichment of Bacterial mRNA from Host-Bacteria Total RNA Samples
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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