QIAamp DNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Protocol A: Using QIAamp DNA mini kit, with prior chemical treatment. In this protocol, we introduced a chemical treatment with sodium dodecyl sulphate and/or β-mercaptoethanol, before commercial kit extraction step.

Protocol B: QIAamp DNA mini kit with glass beads. Extraction was performed using a modification of protocol A, where the chemical treatment step was replaced with boiling for 10 min followed by freezing the sample at −50°C and agitation with 0.5 mm glass beads. Qiagen buffer ATL was added to each tube and a volume of sterile glass beads approximately equal to 100 µl was added. Samples were processed for 30 s pulse at 5000 rpm in a Mini-BeadBeater, followed by centrifugation at 13,200 rpm in a centrifuge for 10 min.

Protocol C: This protocol used completely automated QIAcube extraction using QIAamp DNA mini kit. For this protocol, the initial concentration of the sample was fixed 0.5–1.0 McFarland standard.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

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