SV Total RNA Isolation System

RNA isolation / purification Cells - immortalized MDCK

Experiment
RNA isolation / purification Cells - immortalized MDCK
Product
SV Total RNA Isolation System from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Publication protocol

Total RNA was extracted using the SV total RNA isolation system (Promega) in accordance with the manufacturer’s instructions. Equal amounts of RNA (500 ng) were used to synthesize the first-strand cDNA using the reverse transcriptase system and then PCR. Primers used for PCR were 5′ TCAGTGCAATTGGTGGAAAA 3′ (forward) and 5′ ACCTTGAGCGGAAACCAGTA 3′ (reverse) for CerS1, 5′ CCCACCTTGGAGCATTTCTA 3′ (forward) and 5′ TCGGGTGATGATGAAGACAA 3′ (reverse) for CerS2, 5′ GATT­TTGGCTTCCTCCAACA 3′ (forward) and 5′ GGGGTAGCCATTCCATACCT 3′ (reverse) for CerS3, 5′ TCCTACAGCTCCAACCTGCT 3′ (forward) and 5′ CTGGGCAATGGAGTCGTAGT 3′ (reverse) for CerS4, 5′ GTTCTG­GGACATCCGACAGT 3′ (forward) and 5′ TGAGCTGCTTTCCACATCAC 3′ (reverse) for CerS5, 5′ TAGCCAAACCATGTGCCATA 3′ (forward) and 5′ TGCCTTGTATTCCACAACCA 3′ (reverse) for CerS6, and 5′ ACCACAGTCCATGCCATCAC 3′ (forward), and 5′ ATGTCGTTGTC­CCA­CCACCT 3′ (reverse) for GAPDH. RT-PCR products were resolved on 2% agarose gels.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized MDCK using SV Total RNA Isolation System from Promega.

Paper title
Changes in ceramide metabolism are essential in Madin-Darby canine kidney cell differentiation
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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

Download PDF Download manufacturer protocol

Videos

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