QIAamp DNA Blood Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

2.1.4. DNA extraction and qPCR detection assay
The following protocol was used to analyze the genomic DNA (gDNA) for the yeast stability study and interlaboratory study, and to generate the regression model. gDNA from NE095 cells was extracted using 200 μl (800 cells, 8 × 104 cells, or 8 × 106 cells for low, intermediate, and high concentrations, respectively) aliquots of cells and a QIAamp DNA Blood Mini Kit (cat# 51104 QIAGEN, USA). The spin protocol for DNA purification from blood or body fluids was followed according to the manufacturer’s directions with a few modifications. DNA was incubated at 56 °C for 1 h following the addition of protease and lysis buffer. Extracted DNA was eluted in 200 μl elution buffer provided in the DNA extraction kit and stored at 4 °C prior to downstream analysis.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Blood Mini Kit below.

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