GenElute™ Mammalian Total RNA Miniprep Kit

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	Total RNA was isolated from A2780 and A2780CP20 ovarian cancer cell lines with the GenElute Mammalian Total RNA Purification Kit (Sigma-Aldrich). The RNA concentration was read in a nanodrop. RNA quality was verified in a Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA). One hundred nanograms of total RNA were used to cDNA synthesis.

Protocol tips
Total RNA was isolated from A2780 and A2780CP20 ovarian cancer cell lines with the GenElute Mammalian Total RNA Purification Kit (Sigma-Aldrich). The RNA concentration was read in a nanodrop. RNA quality was verified in a Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA). One hundred nanograms of total RNA were used to cDNA synthesis.

Publication protocol

Total RNA was isolated from A2780 and A2780CP20 ovarian cancer cell lines with the GenElute Mammalian Total RNA Purification Kit (Sigma-Aldrich). The RNA concentration was read in a nanodrop. RNA quality was verified in a Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA). One hundred nanograms of total RNA were used to cDNA synthesis. Complementary cDNA was synthesized, labeled, fragmented and hybridized to the Affymetrix GeneChip Gene 1.0 ST Human Array. After 16 hours of incubation at 45°C, the arrays were washed, stained and scanned (Affymetrix Model 3000 scanner), and data was analyzed using the Partek Genomics Suited (Partek, St. Louis, MO). Experiments were performed in duplicate. Two-way ANOVA was used to determine differences in gene expression (p<0.05 fold change 2). 1359 probes (4%) sets showed ±2-fold change between A2780CP20 and A2780 cells. Additional filtering (±3-fold change, and p<0.003) showed 321 probes (0.96%) changed between A2780CP20 and A2780 cells.

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Download the product protocol from Sigma-Aldrich for GenElute™ Mammalian Total RNA Miniprep Kit below.

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