RNA-Bee

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- cells were directly lysed in culture flasks with RNA-Bee to avoid changes in expression due to harvesting or washing. - Adjust buffers to pH to 6.5 - 7.5 if they have have an acidic pH for better results.	- An additional wash with 75% ethanol improves 260/280 ratio

Upstream tips
- cells were directly lysed in culture flasks with RNA-Bee to avoid changes in expression due to harvesting or washing. - Adjust buffers to pH to 6.5 - 7.5 if they have have an acidic pH for better results.
Protocol tips
- An additional wash with 75% ethanol improves 260/280 ratio

Publication protocol

The NucleoSpin kit (Machery-Nagel) was used according to the manufacturer's protocol to isolate DNA from cells harvested with trypsin and washed with PBS.

For the isolation of total RNA (including microRNAs), exponentially growing cells were directly lysed in culture flasks with RNA-Bee to avoid changes in expression due to harvesting or washing. Total RNA was isolated from lysates. Three independent isolates of RNA from each line were pooled for mRNA and microRNA expression analysis to decrease possible bias from outlier values.

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Manufacturer protocol

Download the product protocol from Amsbio for RNA-Bee below.

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